Real-Time PCR Probes, qPCR probes, TaqMan Probes, dual-label probes

For detailed description of the Real-Time PCR also known as qPCR, please click here (you will be redirected to Wikipedia); for the theory on the TaqMan probes click here (also Wikipedia).  Both types of probes are in fact dual-label probes, whereby the 5'-end is labeled with a reporter dye (such as FAM or Cy5) while the 3'-end is labeled with a quencher (such as the BHQ, MDQ, or TAMRA).

At Alpha ADN, we offer a large number of original and/or replacement reporter dyes, as shown in the table below.  We also offer three types of quenchers: (1) TAMRA as a quencher for FAM. (2) The Black Hole Quenchers (from LGC Biosearch Technologies) or shortly BHQ-1, BHQ-2 and BHQ-3 as quenchers for most dyes used in Real-Time PCR.  (3) MGB (minor groove binder, also known as MGB-NFQ, as it is a non-fluorescent quencher) as quenchers for most dyes used in Real-Time PCR (qPCR).

The table below is not exhaustive, if you do not see the reporter-quencher combination that you are interested in, please inquire with us. 

Please note that some of the reporter dyes in the dual-label probes are priced differently from the same dyes when used in single-label probes.  In a single-label probe, the reporter dye is usually coupled to the 5'-end of the oligonucleotide/probe (similarly to the dual-label probes) but the 3'-end lacks a quencher. The synthesis of the single-label probes can be done in two steps: (a) the DNA part of the oligo is always synthesized on the machine (the DNA synthesizer), but then (b) the reporter dye (a.k.a. the fluorophore, or simply the dye) can be added post-synthetically, after the cleavage of the oligo from the solid support.  This type of modification allows the use of less expensive reagents.  This is the reason why our prices for most fluorescent dyes are quite low, if you look at the Web page with the prices for the single-label dye modifications here.  By contrast, the synthesis of the dual-label probes does not allow the 5'-end reporter dye to be added post-synthetically, for the dual label (reporter-quencher) probes, the 5'-end dye MUST be added on the machine (the DNA synthesizer), which limits our choice of reagents; we are obliged to use more expensive reagents, and as a result, some of the reporter dyes, within the context of a dual-label probe, may cost significantly more than the same reporter dyes in the context of a single-label probe.

Pricelist for some of the available Real-Time PCR / qPCR / TaqMan / dual-label probes

All probes below have the following structure:  "Reporter Dye – 5'-your_oligo_sequence_here-3' – Quencher".  For reporter dyes and quenchers not on the list, please inquire by e-mail to info@alphaadn.com.

Fluorescein---BHQ1, 100 nmol scale

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